Minia University, Faculty of Pharmacy
Journal of advanced Biomedical and Pharmaceutical Sciences
2535-1869
2535-2040
2
2
2019
04
01
Optimization of azo-keratin hydrolysis by alginate-immobilized Keratinase produced from Bacillus licheniforms
41
46
EN
Hesham
M.
El-Komy
Department of Biology, Faculty of Science, Imam Abdulrahman Bin Faisal University, Saudi Arabia
mfarrag83@yahoo.com
Sahar
K.
Al-Dosary
Department of Biology, Faculty of Science, Imam Abdulrahman Bin Faisal University, Saudi Arabia
Mohamed
A.
El-Naghy
Department of Botany and Microbiology, Faculty of Science, Minia University, 61519 Minia, Egypt
Mohamed
A. A.
Abdelhamid
Department of Botany and Microbiology, Faculty of Science, Minia University, 61519 Minia, Egypt
Mohamed
M.
Immam
Department of Botany and Microbiology, Faculty of Science, Minia University, 61519 Minia, Egypt
10.21608/jabps.2019.6237.1029
Recently, much attention has been paid to Keratinolytic materials which can be converted into feed-stuffs, biofertilizers, glues, and foils or used for production of amino acids. Here, we determined the optimum conditions for keratin hydrolysis by alginate-immobilization of crude keratinase isolated from Bacillus lichenoformis. The results of this study indicated that the optimum pH for keratin degradation by immobilized keratinase enzyme was pH 8. Moreover, the crude enzyme was stable for 2 hours at pH 4, 7 and 10 and retained 55.5%, 60% and 53%, of its activity respectively for immobilized enzyme while free enzyme retained only 40%, 40% and 61%, respectively. The optimum temperature for keratinase activity was 50 oC when immobilized in alginate. At temperatures 30, 45 and 60 oC, keratinase was stable for 2 hours and retained 57%, 66% and 57%, of its activity respectively for immobilized enzyme while free enzyme retained only 51%, 62% and 51%, respectively. Furthermore, the effects of activators and inhibitors on the enzyme activity have been investigated.
Keratinase,Bacillus lichenoformis,Immobilization,azo-keratin
https://jabps.journals.ekb.eg/article_23888.html
https://jabps.journals.ekb.eg/article_23888_272fd4557aa920fe0f113b763d503a93.pdf
Minia University, Faculty of Pharmacy
Journal of advanced Biomedical and Pharmaceutical Sciences
2535-1869
2535-2040
2
2
2019
04
01
Two smart spectrophotometric methods for simultaneous determination of Lisinopril and Hydrochlorothiazide in binary mixtures
47
53
EN
Fatma
F.
Mohammed
Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
ph.fatmafarouk@yahoo.com
Khalid
Badr El-Din
Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
kh.badr77@gmail.com
Sayed
M.
Derayea
Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
sayed_derayea@yahoo.com
10.21608/jabps.2019.6766.1031
Two new, simple, fast and sensitive spectrophotometric methods were developed for analysis of binary mixtures of Lisinopril (LIS) and hydrochlorothiazide (HCT). These methods were absorptivity factor method and ratio subtraction method. In the first method, calculations were made using the absorbance of the mixtures at 211 nm where LIS absorptivity is half HCT absorptivity. On the other hand, the second method was based on reobtaining the original spectrum of LIS from the zero order spectrum of the binary mixture then determining the concentration of LIS from its linear regression equation. HCT concentration in both methods was determined from the absorbance at 270 nm where a lack of interference of LIS occurs. The methods were validated according to ICH and applied successfully on different ratios of laboratory mixtures and on pharmaceutical tablets with excellent accuracy and precision.
Spectrophotometric,lisinopril,Hydrochlorothiazide,Absorptivity factor,ratio subtraction
https://jabps.journals.ekb.eg/article_26175.html
https://jabps.journals.ekb.eg/article_26175_218b4be7e47733dbe115dcba12cd4135.pdf
Minia University, Faculty of Pharmacy
Journal of advanced Biomedical and Pharmaceutical Sciences
2535-1869
2535-2040
2
2
2019
04
01
Efficacy and safety of liquorice extract in asthmatic patients
54
58
EN
Eman
Mohammed
Sadek
0000000254476034
Department of Clinical pharmacy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
ems_h84@yahoo.com
Nezar
Rifaat
Tawfik
Department of Chest, Faculty of Medicine, Minia University, 61519 Minia, Egypt
nezarrifaat@yahoo.com
Amal
Kamal
Hussein
Department of Pharmaceutics, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
amal_ka@yahoo.com
Mohammed
Abdelrazek
Abdelhakeem
Department of Clinical pathology, Faculty of Medicine, Minia University, 61519 Minia, Egypt
mat_razek@hotmail.com
10.21608/jabps.2019.6609.1030
Liquorice is one of the commonly used herbs in the field of medicine due to its pharmacological activities. Among these activities, the anti-inflammatory and antiallergic activities that support its use in asthma. This study included 80 asthmatic patients who were classified into two groups, group 1(Placebo group) maintained on inhaled corticosteroids (ICs in moderate to high doses) and long-acting beta agonist (LABA) and received starch capsule (500mg starch) twice daily as placebo and group 2 (active treatment group) maintained on ICs (in moderate to high doses) and LABA and received liquorice extract capsule (500mg equivalent to 100mg glycyrrhizin) taken twice daily. The efficacy of liquorice was measured by estimation of pulmonary function and blood eosinophils %. On the other hand, safety was assessed by blood pressure measurement and determination of serum potassium level. The results revealed that addition of liquorice capsules to ICs and LABA resulted in a non-significant improvement in blood eosinophils (P-value 0.754). However, it resulted in a highly significant improvement in Forced Vital Capacity (FVC) % and Forced Expiratory Volume1 (FEV1) % when compared to group 1 (P-value 0.031.and 0.040 respectively). Regarding liquorice safety, neither Blood pressure (systolic and Diastolic) (SBP and DBP), nor serum K level showed any significant change in patients received liquorice capsules. Conclusion: liquorice in the used dose significantly improved FVC% and FEV1% but did not affect blood eosinophils. There was no significant effect on blood pressure and serum K level, so, this dose is safe with no observed side effects.
Asthma,Liquorice,Glycyrrhizin,pulmonary function
https://jabps.journals.ekb.eg/article_28312.html
https://jabps.journals.ekb.eg/article_28312_f5e8912df06c48ca5dcf0f3c13d64841.pdf
Minia University, Faculty of Pharmacy
Journal of advanced Biomedical and Pharmaceutical Sciences
2535-1869
2535-2040
2
2
2019
04
01
Natural products potential of the genus Aptenia
59
62
EN
Asmaa
Abo Elgoud
Said
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
rabouelgoud@yahoo.com
Eman
Zekry
Attia
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
eshihata@yahoo.com
Usama
Ramdan
Abdelmohsen
0000-0002-1014-6922
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
usama.ramadan@mu.edu.eg
Mostafa
Ahmed
A. Fouad
0000-0002-3909-3537
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
m_fouad2000@yahoo.com
10.21608/jabps.2019.6908.1032
Plants are considered rich attractive sources for producing new bioactive compounds which exhibit a wide range of pharmacological activities. Family Aizoaceae is considered to be one of the South Africa largest succulent plant families, and it consists of four subfamilies named Sesuvioideae, Aizooideae, Ruschioideae, and Mesembryanthemoideae. Genus Aptenia consists of four species such as Aptenia cordifolia, Aptenia geniculiflora, Aptenia haeckeliana, and Aptenia lancifolia. Along Aptenia species are rich sources of natural metabolites from diverse chemical classes including oxyneolignans, flavonoids, sterols, lignans, amides and alkaloids with powerful anti-inflammatory activity. This review covers the literature until December 2018, providing a complete survey of all compounds isolated from genus Aptenia with their corresponding biological activities whenever applicable.
Aptenia,Alkaloids,lignans,amides,Anti-inflammatory
https://jabps.journals.ekb.eg/article_28382.html
https://jabps.journals.ekb.eg/article_28382_113e2500d111d3bb7bd2a2af5398f89d.pdf
Minia University, Faculty of Pharmacy
Journal of advanced Biomedical and Pharmaceutical Sciences
2535-1869
2535-2040
2
2
2019
04
01
Novel chemo-fluorimetric methods for determination of bambuterol hydrochloride in presence of the active metabolite terbutaline
63
71
EN
Khalid
M. Badr
El-Din
Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
kh.badr77@gmail.com
10.21608/jabps.2019.9819.1035
Three novel, simple, rapid and sensitive spectrofluorimetric methods were developed for the simultaneous determination of bambuterol hydrochloride (BAM) in presence of its active metabolite and acidic degradation product; terbutaline (TER). Method I based upon calculating the first derivative values (D1) of the previously stored conventional emission spectra of both drugs. BAM and TER were measured at 302 and 286 nm respectively. Methods II & III built upon estimating the sum amount of both drugs at the isosbestic points (287.5 nm in the conventional emission method and 229.5, 247.5 nm in synchronous emission method). TER was determined using the previously mentioned D1 methods while BAM concentrations were considered by subtraction. All the optimization parameters were carefully optimized such as Δ λ and diluting solvents. The calibration curves were rectilinear over the range of 0.4 – 8.0 µg mL-1 with quantitation limits ranged from 0.246 – 0.273 µg mL-1. The proposed methods were successfully applied to the assay of synthetic laboratory mixtures, commercially available tablets as well as forced acidic stability testing.
Bambuterol,Synchronous,Spectrofluorimetry,Isosbestic point,First derivative
https://jabps.journals.ekb.eg/article_28566.html
https://jabps.journals.ekb.eg/article_28566_b6a3d31538f58d605df3516802171718.pdf
Minia University, Faculty of Pharmacy
Journal of advanced Biomedical and Pharmaceutical Sciences
2535-1869
2535-2040
2
2
2019
04
01
Promising Antidiabetic and Wound Healing Activities of Forsskaolea tenacissima L. Aerial Parts.
72
76
EN
Hamdy
K.
Assaf
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, 71524 Assiut, Egypt
hamdyss200@yahoo.com
Alaa
M.
Nafady
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, 71524 Assiut, Egypt
alaa12nafady@yahoo.com
Ahmed
E.
Allam
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, 71524 Assiut, Egypt
aallam81@yahoo.co.uk
Ashraf
N. E.
Hamed
0000-0003-2230-9909
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
ashrafnag@mu.edu.eg
Mohamed
S.
Kamel
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
mskamel2@gmail.com
10.21608/jabps.2019.7542.1034
The aim of present study was to evaluate antidiabetic activity of the total methanolic extract (TME) and different fractions of Forsskaolea tenacissima L. aerial parts with alloxan induced diabetic rats and evaluate wound healing activity with excision wound model in rats as well as determination of toxicological activity by lethal dose 50% (LD50). The ethyl acetate fraction showed a significant decrease in blood glucose level (36.00%, P<0.001) in comparison with glibenclamide (36.14%, P<0.001) as standard. The TME group showed marked increase in wound healing activity (97.9%, P<0.001) compared with Garamycin® ointment standard group (96.8%, P<0.001). The TME is safe due to the LD50 was 7 g/kg body weight.
Forsskaolea tenacissima,Urticaceae,antidiabetic,Wound healing,Acute toxicity
https://jabps.journals.ekb.eg/article_28759.html
https://jabps.journals.ekb.eg/article_28759_634a6cbda0d344edddc11df9157cceff.pdf