Minia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693420201001Botanical studies on the stem and root of Melissa officinalis L. (lemon balm)1841899881210.21608/jabps.2020.29774.1085ENWaleed A.Abdel-NaimeDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, EgyptJohn R.FahimDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt0000-0002-2425-0819Mostafa A.FouadDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt0000-0002-3909-3537Mohamed S.KamelDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Deraya University, 61111 New Minia, EgyptJournal Article20200511Melissa officinalis L. (family Lamiaceae) is a perennial herbaceous plant naturally found in southern Europe and central Asia, with worldwide culinary and medicinal applications. It shows an erect, quadrangular stem that grows up to 1.5 m in height, carrying simple ovate to cordate leaves in an opposite decussate pattern. Microscopically, M. officinalis is generally characterized by anomocytic stomata as well as a variety of non-glandular and glandular trichomes, exemplified by labiaceous and capitate hairs; giving the plant a hairy texture and distinctive lemon-like scent thanks to the considerable secretion of volatiles from the latter hair types. Additionally, different tissues of this herb are generally devoid of calcium oxalate crystals. As part of our ongoing phytochemical and botanical research on M. officinalis, which previously included the detailed macroscopical and microscopical analysis of its leaves, the present study describes the botanical features of stems and roots of M. officinalis. Such botanical data could be of value as standardized parameters for authentication purposes of this multipurpose plant species.https://jabps.journals.ekb.eg/article_98812_bd35fea8964d37b00795b833c69fba04.pdfMinia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693420201001Silymarin protects against sepsis-induced acute liver and kidney injury via anti-inflammatory and antioxidant mechanisms in the rat19019711174110.21608/jabps.2020.37074.1091ENAlaa Al-KadiDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Deraya University, El-Minia, EgyptAl-Shaimaa AhmedDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, El-Minia, Egypt0000-0001-9875-639Nashwa Fathy GamalEl-TahawyDepartment of Histology and Cell biology, Faculty of Medicine, Minia University, El-Minia, EgyptMohamed Montaser A.KhalifaDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, El-Minia, EgyptMahmoud El-DalyDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, El-Minia, Egypt0000-0001-5971-1104Journal Article20200724Sepsis is a leading cause of death among intensive care patients. During sepsis, exaggerated reaction to infection leads to massive production of reactive oxygen species and inflammatory mediators, which eventually leads to multiple organ damage. Silymarin is a well-known antioxidant and cytoprotective agent, which showed protective effects in different models of disease. Thus, we hypothesized that silymarin would be protective against sepsis-induced liver and kidney injury. Sepsis was induced in rats by the cecal ligation and puncture (CLP) method. Rats were divided into sham, CLP-non-treated and CLP treated with silymarin (100 mg/kg, i.p. 1 h following CLP). After 24 h, rats were euthanized for blood and tissue samples, which were used for assessment of MDA, NO, GSH, IL-6 and TNF-α levels and SOD activity, in addition to renal and hepatic function parameters. Survival study was conducted using another set of animals following the same previously mentioned procedure. Silymarin showed protective effects evidenced by enhanced overall survival following sepsis (80% in silymarin-treated vs. 20% in septic group), in addition to improvement of hepatic and renal function parameters and reduction of MDA, NO, IL-6 and TNF-α levels. Moreover, silymarin supported the endogenous antioxidant mechanisms via elevation of GSH levels and reinforcement of SOD activity. In conclusion, silymarin protects against sepsis-induced hepatic and renal injury, possibly via antioxidant and anti-inflammatory mechanisms.https://jabps.journals.ekb.eg/article_111741_80c7f1c4b6bfe84410bccfe85dfa433b.pdfMinia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693420201001Macro- and microscopical characterization of the stem and flowers of Malvaviscus arboreus Cav. (Malvaceae)19820511248810.21608/jabps.2020.31092.1088ENOmnia H.AbdelhafezDepartment of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia 61111, EgyptJohn R.FahimDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt0000-0002-2425-0819Usama R.AbdelmohsenDepartment of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia 61111, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt0000-0002-1014-6922Samar Y.DesoukeyDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt0000-0002-4462-9674Journal Article20200527Malvaviscus arboreus Cav. (Sleeping Hibiscus) is a species of flowering plants belonging to family Malvaceae with a natural distribution throughout Central and South America. However, this perennial, medium-sized shrub has been also widely cultivated in several other tropical and subtropical areas of the globe. Sleeping Hibiscus is characterized by an erect, cylindrical, and glabrous stem with monopodial branching, reaching up to 0.5‒1 m in height and 0.2‒1 cm in diameter. The stem carries simple, alternate, oval or ovate to broadly cordate leaves and red solitary flowers. Flowers of Sleeping Hibiscus are tube-shaped with totally wrapped petals that only slightly open at the top, carried on hairy, pendulous green pedicels. Besides their ornamental value, M. arboreus plants enjoy multiple folk, medicinal, and culinary applications worldwide owing to their wide range of bioactive secondary metabolites, principally phenolics. Hence, in continuance of our interest in this medicinal plant, which included formerly the comprehensive botanical description of its leaves, along with the phytochemical and biological characterization of its leaf and stem extracts, this paper presents the macro- and microscopical characters of M. arboreus stems and flowers. Such botanical data collectively draw a helpful image regarding the identification and authentication of this plant species.https://jabps.journals.ekb.eg/article_112488_7220c46efac97c8403b18dbc490f4575.pdfMinia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693420201001Simple development and validation of RP-HPLC and TLC- densitometric methods for the simultaneous determination of nadifloxacine and mometasone furoate in their binary mixture20621211365410.21608/jabps.2020.37622.1093ENOla M.AbdallahaAnalytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University (Girls), Cairo, Egypt.
Pharmaceutical Chemistry Department, Faculty of Pharmacy, Egyptian Russian University in Egypt (ERU), Badr City, Cairo, Egypt.Ahmed M.Abdel-MegiedeAnalytical Chemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh City, EgyptMona A.AbdelrahmanPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, October University, 6th October City, Giza, EgyptShimaa A.AbdelatteyPharmaceutical Chemistry Department, National Organization for Drug Control and Research (NODCAR), Giza, Egypt.Journal Article20200729The present work is concerned with development, optimization and validation of two chromatographic methods for the simultaneous determination of nadifloxacine (ND) and mometasone furoate (MF). The first developed method was RP-HPLC depended on chromatographic separation using Phenyl-hexyl column and a mobile phase composed of acetonitrile: acidified water with orthophosphoric acid up to (pH 2.5 ± 0.1) in the proportion of (65: 35 v/v) pumped at a flow rate of 1.25 mL min−1. All measurements were performed with UV detection at 254 nm. The second method was TLC-densitometry, chromatographic separation was established on aluminum TLC plates pre-coated with silica gel GF254 as the stationary phase and chloroform: methanol: hexane: ethyl acetate: acetic acid (9: 1: 3: 3: 0.1, by volume) as the mobile phase followed by densitometric measurement of the separated bands at 254 nm. Validation of the suggested methods was successfully applied with respect to ICH guidelines. The proposed chromatographic methods were used to determine both drugs binary mixture in pure form and dosage form. The proposed methods give good linearity in the range 0.5‒5.0 μg/mL and 0.5‒40 µg/spot for HPLC and HPTLC methods, respectively. While MF standard solutions in the range 0.2‒1.2 μg/mL and 0.5‒3.8 µg/ spot for HPLC and HPTLC methods, respectively. The obtained results were statistically compared with those achieved by the reported methods, showing no significant difference with respect to accuracy and precision at p = 0.05.https://jabps.journals.ekb.eg/article_113654_302a6fc3a21a20a07328535127608576.pdfMinia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693420201001Phytochemical investigation of saponifiable matter & volatile oils and antibacterial activity of Moluccella laevis L., family Lamiaceae (Labiatae)21322011527110.21608/jabps.2020.38933.1096ENAshraf HamedDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, El-Minia 61519, Egypt0000-0003-2230-9909Nousiba AhmedDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, El-Minia 61519, EgyptEman AttiaDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, El-Minia 61519, EgyptSamar DesoukeyDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, El-Minia 61519, Egypt0000-0002-4462-9674Journal Article20200815<strong>Abstract</strong><br /> <br /> The current study was aimed to evaluate some parts of <em>Moluccella laevis viz</em>., phytochemical and antibacterial. Regarding to GC/MS of the saponifiable matters of petroleum ether fraction of total ethanolic extract of the aerial parts (TEE), the main recognized unsaturated fatty acids were methyl linolenate (25.58%) and methyl linoleate (15.87%). Whereas, the major saturated fatty acids were, palmitic (25.4%) followed by stearic acid (10.48). A comparative analysis of the volatile constituents of <em>M. laevis</em> flowers and leaves was performed by Head Space GC/MS. The volatile mixtures of both plant parts displayed comparable amounts of hydrocarbons and oxygenated compounds, with a noticeable greater contribution of the latter in the leaves.Besides, <em>a</em>-pinene (40.84%), chrysanthenyl acetate (17.89%) and isobornyl acetate (10.64%), were identified as the major volatile components in the flowers. While, isobornyl acetate (35.09%) was characterized as the major constituent followed by, 2-methyl-4-butanolide (22.12%), 1-heptene oxide (7.47%) and benzoic acid, methyl ester (4.05%) of the volatile oil composition of the leaves. Moreover, this study included the antibacterial activity of TEE and its different fractions against Gram positive and negative bacteria. TEE exhibited MIC values of 326 476, and 541 µg/mL against <em>E. coli, K. pneumonia</em> and <em>P.</em><em> aeruginosa, </em>respectively, while the aqueous fraction showed MICs of 410, 633, 748 and 10713 µg/mL against <em>P. aeruginosa</em>, <em>K. pneumonia</em>, <em>E. coli </em>and <em>S. aureus</em>, respectively. Finally, the EtOAc fraction displayed MICs 449, 541 and 1085 µg/mL against <em>K. pneumonia, E. coli</em> and <em>P. aeruginosa</em>, respectively.https://jabps.journals.ekb.eg/article_115271_76269fb17b22e9b3d0b2ecb24e05f477.pdf