Minia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693120200101Cubosomes: composition, preparation, and drug delivery applications.195478110.21608/jabps.2019.16887.1057ENSherif A.GaballaDepartment of pharmaceutics, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt0000-0001-5916-9343Omar H.El GarhyDepartment of pharmaceutics, Faculty of Pharmacy, Minia University, 61519 Minia, EgyptHamdy AbdelkaderDepartment of pharmaceutics, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt0000-0003-4989-5143Journal Article20190911Abstract <br /> Cubosomes can be considered as a novel lipid-based nanosystems similar to well-known vesicular systems such as liposomes and niosomes. Cubosomes have been widely formulated using certain amphiphilic lipids (e.g. glyceryl monooleate and phytantriol) in the presence of a suitable stabilizer. They can represent a novel drug delivery system which could be loaded with hydrophilic, lipophilic and amphiphilic drug molecules. They are widely used for various drug delivery applications such as oral, ocular, transdermal and chemotherapy drug delivery. In this review, the pertinent literature of cubosomes with emphasis on theories of self-assembling, the composition of cubosomes, methods of preparation and drug delivery applications will be critically reviewed.Minia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693120200101Preparation and evaluation of Ketotifen suppositories10226617710.21608/jabps.2019.19318.1059ENDina F. M.MohamedDepartment of Pharmaceutics, Faculty of Pharmacy, Assiut University, 71516 Assiut, Egypt.Omnia A. E.MahmoudDepartment of Pharmaceutics, Faculty of Pharmacy, South Vally University, 83523 Qena, Egypt.Fergany A.MohamedDepartment of Pharmaceutics, Faculty of Pharmacy, Assiut University, 71516 Assiut, Egypt.Journal Article20191111Ketotifen KT is one of antiallergic drugs, due to its first pass effect, the bioavailability of the drug is only 50%. The objective of this study was to formulate and evaluate suppositories containing KT and/or KT solid dispersion. <br /> The in-vitro release of KT from suppositories was done using dialysis membrane method in phosphate buffer at pH 7.4. The release of KT from water soluble suppository bases was higher than that from fatty or emulsion suppositories bases. Among all PEGs bases (F4: PEG 6000: PG (20: 80)) showed a relatively higher release of KT. Formulations prepared with glycerin bases gave more or less identical release pattern; relatively formula (F17: Gelatin: Glycerin: Propylene glycol: Water) gave the highest release pattern. Formula (F20: Suppocire AM) exhibited the highest release rate among fatty bases. Within all emulsion bases (F23: W15: W75: Tween 20: Span 60: PEG 1500: Propylene glycol) showed highest release rate. KT solid dispersion led to a higher release rate of the drug from selected bases. <br /> A histological comparison between control group of rabbits (didn`t take suppository), another group took plain suppositories and group that received suppositories containing solid dispersion of KT was carried out. The tested plain and medicated bases didn’t injure the rectal mucosa of rabbits. In conclusion the incorporation of solid dispersion in formula (F4) complied with the pharmacobeial limits for hardness, dissolution time, content uniformity and weight variation. Also it showed a relatively higher in-vitro release of KT and considered as safe and useful formulation for clinical use.Minia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693120200101Fast disintegrating tablets of spray dried poly-herbal extract: a promising hypoglycemic and diabetic wound healing activity.23306618610.21608/jabps.2019.19840.1060ENMohamed MahranDepartment of Pharmaceutics, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.Omar HelmyElgarhyDepartment of Pharmaceutics, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.Amal HusseinDepartment of Pharmaceutics, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.Al-Shaimaa F.AhmedDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.0000-0001-9875-639Mohamed Ahmed SharaweTahaDepartment of Internal Medicine and Nephrology, Faculty of Medicine, Minia University, 61519 Minia, Egypt.Eman AlaaeldinDepartment of Pharmaceutics, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.Department of Clinical Pharmacy, Faculty of Pharmacy, Deraya University, 61111 New Minia, Egypt.Journal Article20191119Many herbal products have been used to control blood glucose level and minimize complications of diabetes mellitus. However, the feasibility of using such herbal product has been hindered due to the lower stability of their extracts and patients incompliance.<br /> Objective: An optimized fast disintegrating tablet preparation of spray dried aqueous extract of poly herbal blend has been formulated to maximize the therapeutic activity of such herbal extract.<br /> Methods: different ratios of superdisintegrants were evaluated in terms of reducing disintegration time and wetting time of the prepared formulations. The potential of the optimized formula in reducing blood glucose level and promoting wound healing in STZ-induced diabetic rats was determined.<br /> Key finding: Oral administration of the optimized formula (F6) had significant superior effect in reducing blood glucose level and promoting wound healing of diabetic rats compared receiving the aqueous extract of the herbal blend (P<0.05, p<0.01, respectively). That may be attributed to the enhanced stability and the accurate dosing of such solid dosage form. In addition, no remarkable toxic manifestations or histologic changes were observed in treated animals.<br /> Conclusion: These findings uncover the potential of the formulated dosage form in enhancing the hypoglycemic and wound healing activity of the herbal extract.Minia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693120200101Amelioration of Sepsis-Induced Liver and Lung Injury by a Superoxide Dismutase Mimetic; Role of TNF-α and Caspase-331396619410.21608/jabps.2019.19876.1061ENAl-Shaimaa F.AhmedDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.0000-0001-9875-639Asmaa M.A.BayoumiDepartment of Biochemistry, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.0000-0001-6757Heba M.EltahirDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Taibah University, Saudi Arabia.Sara M.N.Abdel HafezDepartment of Histology and Cell Biology, Faculty of Medicine, Minia University, 61519 Minia, Egypt.Mekky M.AbouziedDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Taibah University, Saudi Arabia.Journal Article20191120Oxidative stress plays an important role in the development of sepsis and its associated serious consequence leading to multiple organ failure and death. Since the liver and the lungs are among the early affected organs responsible for the mortality in sepsis, we investigated the effect of Tempol, a superoxide dismutase mimetic agent, on lung and liver injuries in a cecal ligation and puncture (CLP)-induced sepsis. Septic animals were given Tempol either before or after CLP procedure. Sepsis outcomes were assessed mainly on the liver and lungs. Separate animal groups were employed for a survival study. CLP resulted in 0% survival, while Tempol pre-or post-treatment led to a 100% and 40% survival, respectively. Administration of Tempol resulted in a significant attenuation of sepsis-induced elevation of lipid peroxidation. In the lungs and liver tissues, Tempol resulted in a significant attenuation of elevated tumor necrosis factor-α and caspase-3. Histopathological examination of the lungs and liver confirmed the protective effects of Tempol on these organs. In conclusion: Tempol acts as both prophylactic and therapeutic agent in a rat sepsis model by lowering oxidative stress, inflammatory and apoptotic signals induced by sepsis and reducing lung and liver damage induced by sepsis.Minia University, Faculty of PharmacyJournal of advanced Biomedical and Pharmaceutical Sciences2535-18693120200101Survey the Genetic Diversity of Eight Opuntia Species from Egypt using Random Amplified Polymorphic DNA (RAPD) Technique40446594010.21608/jabps.2019.20728.1062ENMohamed RabehDepartment of Pharmacognosy, Faculty of pharmacy, Cairo University, Cairo 11562, Egypt.Seham Salah El-DinEl-HawaryDepartment of Pharmacognosy, Faculty of pharmacy, Cairo University, Cairo 11562, Egypt.o, EgyptMona El-MahdyEl-TantawyDepartment of Medicinal plants and natural products, National Organization of Drug Control and Research, Giza, Egypt.Usama RamadanAbdelmohsenDepartment of Pharmacognosy, faculty of Pharmacy, Minia University, 61519 Minia, Egypt0000-0002-1014-6922Wafaa KoranyBadrDepartment of Medicinal plants and natural products, National Organization of Drug Control and Research, Giza, Egypt.Journal Article20191209Opuntia is a large genus of succulent shrubs characterized by unique attractive flowers known as prickly pears by virtue of their characteristic edible fruits. They are used as laxative, diuretic, antipyretic, and anti-inflammatory. <br /> This study aims to authenticate eight different Opuntia species, Opuntia brasilliensis (Willd.) Haw (O1), Opuntia dillenii (ker Gawl.) Haw. (O2), Opuntia dejecta Salm-Dyck (O3), Opuntia ficus indica (L.) Mill. (O4), Opuntia tomentosa Salm-Dyck (O5), Opuntia phaecantha Engelm. (O6), Opuntia leucotricha DC. (O7), Opuntia microdasys (Lehm.) Pleiff. (O8), growing in Egypt by using random amplified polymorphic DNA technique. Accurate identification of the eight Opuntia species is an urgent need to maintain herbal formulations potency and peculiarity. <br /> Similarity coefficients of 40.0 - 67.6% classified Opuntia species into two main groups, one of them contains two species and the other one contains six species. Consequently, this technique helps the identification of these Opuntia species showing great morphological similarity.