Soil actinomycetes molecular characterization for secondary metabolites production

Ullah, Shakir; Muhammad, Zubaida Sher; Jehan, Saqlain; Zia, Samavia; Hussain, Zakir; Hussain, Syed Amjad; Shirazi, Ramiz Raza; Ullah, Asad; Raziq, Abdur; Gul, Anbareen; Ullah, Hayat

doi:10.21608/jabps.2021.98256.1141

Soil actinomycetes molecular characterization for secondary metabolites production

Document Type : Original Article

Authors

¹ Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Pakistan.

² Department Zoology, Sardar Bahadur Khan Women University Quetta Baluchistan

³ Department of Chemistry, Government College University Faisalabad Pakistan

⁴ Department of Chemistry, The university of Punjab Lahore Pakistan

⁵ Department of Microbiology, Faculty of Life Sciences and Informatics, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, Pakistan.

⁶ Department of Bioinformatics & Biotechnology, Faculty of Life Science, Government College University Faisalabad, Pakistan.

⁷ Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

⁸ Government College University Faisalabad

10.21608/jabps.2021.98256.1141

Abstract

Antibiotic resistance issue is really unavoidable, increased due to decrease in production of novel secondary metabolites production. Actinomycetes have the potential to produce biological active compounds used as an antibacterial, antiviral, antifungal, antiprotozoal, antihelminthic, anticholesterol, anticancer, immunosuppressant, pesticides and herbicides. Current research study was design to assess actinomycetes for production secondary metabolites. For this purpose different soil samples were collected and with help of dilution method, actinomycetes were grown on enrichment. Actinomycetes were morphologically analysed under microscope and molecularly through PCR and 16S-rRNA gene-amplification. After confirmation, actinomycetes were grown on LB and GMC media have potential to produce bioactive secondary metabolites. Current research study results that actinomycetes have potential to produce bioactive secondary metabolites. LB-media is the best suitable-medium for growth of actinomycetes and bioactive secondary-metabolites production. It is suggested that actinomycetes strains from various environment should be assessed for novel bioactive secondary metabolites.

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