Hammad, A., Ahmed, A., Heeba, G., Taye, A. (2019). Resveratrol protects against early cardiomyopathic changes-induced by Type-1 diabetes through a heme-oxygenase-1 dependent mechanism. Journal of Advanced Biomedical and Pharmaceutical Sciences, 2(1), 12-18. doi: 10.21608/jabps.2018.5175.1022
Asmaa S.A. Hammad; Al-Shaimaa F. Ahmed; Gehan H. Heeba; Ashraf A. Taye. "Resveratrol protects against early cardiomyopathic changes-induced by Type-1 diabetes through a heme-oxygenase-1 dependent mechanism". Journal of Advanced Biomedical and Pharmaceutical Sciences, 2, 1, 2019, 12-18. doi: 10.21608/jabps.2018.5175.1022
Hammad, A., Ahmed, A., Heeba, G., Taye, A. (2019). 'Resveratrol protects against early cardiomyopathic changes-induced by Type-1 diabetes through a heme-oxygenase-1 dependent mechanism', Journal of Advanced Biomedical and Pharmaceutical Sciences, 2(1), pp. 12-18. doi: 10.21608/jabps.2018.5175.1022
Hammad, A., Ahmed, A., Heeba, G., Taye, A. Resveratrol protects against early cardiomyopathic changes-induced by Type-1 diabetes through a heme-oxygenase-1 dependent mechanism. Journal of Advanced Biomedical and Pharmaceutical Sciences, 2019; 2(1): 12-18. doi: 10.21608/jabps.2018.5175.1022
Resveratrol protects against early cardiomyopathic changes-induced by Type-1 diabetes through a heme-oxygenase-1 dependent mechanism
1Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
2Department of Pharmacology & Toxicology, Faculty of Pharmacy, South Valley University, Qena, Egypt
Abstract
Diabetes can be considered as a state of chronic inflammation and oxidative stress. Excessive oxidative stress is implicated in the pathogenesis of all diabetic complications. Heme oxygenase-1 is an antioxidant enzyme that is upregulated in response to a variety of insults as a protective mechanism. Resveratrol (RSV) is a polyphenol that exhibits promising pharmacological effects in a variety of disease models. In this study, we investigated the role of RSV in prevention of early pathological changes in the diabetic heart as well as the role of hemeoxygenase-1 in this regard. To achieve this aim, diabetes was induced by ip injection of streptozotocin (50 mg/kg). The rats were divided into 5 experimental groups; normal control (CTR), diabetic control (DM), diabetic rats treated with RSV (DM+RSV); 10 mg/kg) in drinking water, diabetic rats treated with a combination of RSV and an HO-1 inhibitor, Zinc protoporphyrin (ZnPP), (DM + RSV+ZnPP); 10 µmole/Kg/week I.P) and diabetic rats treated with ZnPP alone (DM + ZnPP). Induction of diabetes was accompanied by a significant increase in cardiac MDA level and TGF-β protein expression as well as a significant reduction in eNOS and HO-1 expression. Chronic treatment with RSV significantly attenuated the abovementioned biochemical changes associated with diabetes in addition to alleviation of diabetes-induced histopathological alterations. Furthermore, the effect of RSV was diminished when the HO-1 activity was blocked by ZnPP. These results demonstrate the role of HO-1 in mediating RSV protective effects against diabetes-induced early cardiomyopathy.
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