Phytochemical Screening, and In Vitro Evaluation of Antioxidant Activity of Lolium Perenne

Nagaty Mahrous, Mahmoud; Attia, Eman Zekry; Abdelkader, Mohamed Salaheldin A.; Hamed, Ashraf N. E.

doi:10.21608/jabps.2023.198473.1185

Phytochemical Screening, and In Vitro Evaluation of Antioxidant Activity of Lolium Perenne

Document Type : Original Article

Authors

¹ Department of Pharmacognosy, Faculty of Pharmacy, Sohag University, Sohag, 82524, Egypt.

² Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt

³ Department of Pharmacognosy, Faculty of Pharmacy, Sohag University, Nasr City, Eastern Avenue, P.O. Box 11432, Sohag, Egypt.

⁴ Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia Egypt

10.21608/jabps.2023.198473.1185

Abstract

The current study aimed to investigate the preliminary phytochemical screening, quantify the total phenolic (TPC), flavonoidal (TFC) contents and evaluate the antioxidant activities of the total methanol extract (ME) of the aerial parts and its derived fractions of Lolium perenne L. (L. perenne). The preliminary phytochemical screening of ME displayed the presence of carbohydrates and/or glycosides, steroids and/or triterpenes, phenolic compounds, proteins and/or amino acids, flavonoids, and quinones. Dichloromethane (DFr) and ethyl acetate (EFr) fractions showed the highest TPC (82.42±2.70 and 70.85±2.27 mg gallic acid equivalent (GAE)/g dried sample), respectively. Also, DFr and EFr displayed the highest TFC (115.65±6.43 and 146.00±1.06 mg rutin equivalent (RE)/g dried sample), respectively. All samples showed variable antioxidant properties. While EFr, followed by DFr, demonstrated the least IC50 value of 120.04±0.43 and 166.81±0.53 µg/mL, respectively, indicating their high antioxidant potential.

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