Document Type : Original Article
Authors
1
Department of Pharmacology and Toxicology, College of Pharmacy, Taibah University, Al-Madinah Almunawwarah, Kingdom of Saudi Arabia
2
Department of Biochemistry, Faculty of pharmacy, New valley University, New Valley, El-kharga city 71511, Egypt
3
Department of Biochemistry, Faculty of pharmacy, Deraya University, New Minia city 61111, Egypt
4
Department of hepatology and gastroenterology, Faculty of medicine, Minia University, Minia city 61519, Egypt
5
Department of Biochemistry, Faculty of pharmacy, Alexindria University, Egypt
6
Medical center Taibah University, Medina, Kingdom of Saudi Arabia
7
Department of clinical and hospital pharmacy, College of Pharmacy, Taibah University, Medina, Kingdom of Saudi Arabia
8
Department of Pharmacology and Toxicology, College of Pharmacy, Taibah University, Madinah, Saudi Arabia.
9
Clinical Pharmacy program, Al-Rayan Colleges, Medina, Kingdom of Saudi Arabia
10
Department of faculty of Pharmacy, Minia University, Minia, Egypt
Abstract
Background: Survivin is one of the most crucial apoptosis inhibitory genes, playing a basic role in hepatocellular carcinoma (HCC) development and progression. The current study aims at investigating the relationship of survivin polymorphism with the risk of HCC in Hepatitis C virus-infected Egyptian population.
Methods: the study was conducted on 120 Egyptian individuals divided into: healthy controls, fibrotic patients, cirrhotic patients and HCC patients (30 subjects/group). History and risk factors were collected and recorded for all cases. Polymorphism of the survivin gene, including one locus (rs9904341) was chosen for genotyping using (PCR-RFLP) technique.
Results: No statistically significant difference was detected in the genotype or allele distribution of HCC subjects in respect to the controls (P>0.05). On the other hand, a significant difference could be detected in genotype or allele distribution in fibrosis and cirrhosis HCV patient groups compared to healthy controls (P<0.05). A significant difference in genotype or allele distribution was detected in HCC group compared to cirrhosis group (P<0.05). No statistically significant difference was found in genotype or allele distribution of fibrosis cases relative to the controls.
Conclusion: No relationship between Survivin gene (-31G/C) polymorphism rs9904341 and the risk of HCC could be found in HCV-infected Egyptian population. However, a positive correlation could be detected between Survivin gene polymorphism and the risk of cirrhosis in the same population. The study also identified a relationship between survivin gene polymorphism in cirrhosis relative to fibrosis as well as between survivin gene (–31G/C) polymorphism rs9904341 in HCC relative to cirrhosis.
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