Abd Alhady, M., Abo El-Fadl, R., Hegazi, G., Desoukey, S. (2020). In vitro production of some secondary metabolites from Cupressus sempervirens.. Journal of Advanced Biomedical and Pharmaceutical Sciences, 3(3), 127-134. doi: 10.21608/jabps.2020.24700.1074
Mohamed Reda Abd Almegid Abd Alhady; Reda El-Said Abo El-Fadl; Ghada Abd El-Moneim Hegazi; Samar Yehia Desoukey. "In vitro production of some secondary metabolites from Cupressus sempervirens.". Journal of Advanced Biomedical and Pharmaceutical Sciences, 3, 3, 2020, 127-134. doi: 10.21608/jabps.2020.24700.1074
Abd Alhady, M., Abo El-Fadl, R., Hegazi, G., Desoukey, S. (2020). 'In vitro production of some secondary metabolites from Cupressus sempervirens.', Journal of Advanced Biomedical and Pharmaceutical Sciences, 3(3), pp. 127-134. doi: 10.21608/jabps.2020.24700.1074
Abd Alhady, M., Abo El-Fadl, R., Hegazi, G., Desoukey, S. In vitro production of some secondary metabolites from Cupressus sempervirens.. Journal of Advanced Biomedical and Pharmaceutical Sciences, 2020; 3(3): 127-134. doi: 10.21608/jabps.2020.24700.1074
In vitro production of some secondary metabolites from Cupressus sempervirens.
1Tissue Culture Unit, Department of Genetic Resources, Ecology and Dry Land Agriculture Division, Desert Research Center, 1 Matahaf El-Matareya Street, 11753 El-Matareya, Cairo, Egypt
2Department of Pharmacognosy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
Abstract
Cupressus sempervirens L. has known medicinal activities, which are related to the presence of miscellaneous secondary metabolites, involved in patents for pharmaceuticals and/or cosmetics in the market. The aim of this study is to perform a comparative investigation of the main metabolites in the wild, cultivated and callus cultures of C. sempervirens using different treatments and the in vitro production of certain metabolites from C. sempervirens callus cultures in concentrations adequate for use commercially and also to develop a convenient chromatographic method for both the qualitative and quantitative determination of the most beneficial flavonoids (rutin and quercitrin), along with other metabolites (ferruginol, 2-furancarboxaldehyde,5 methyl, pentadecanoic acid, totarol, quinic acid and hinokiol) using HPLC/DAD and GC/MS, respectively. Leaf extract of the wild plant induced reduction in cell viability of some tested cell lines as: human hepatocellular carcinoma cell (HEPG2), lung carcinoma cell (A549), human Caucasian breast adenocarcinoma (MCF7) and especially on human colon cancer cell (HCT116), which was higher than doxorubicin used as a standard. This study is a stepping stone for obtaining natural and renewable bioactive secondary metabolites from C. sempervirens through in vitro callus cultures.
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